Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast.

AUTOR(ES)

Hannig, E M

RESUMO

The M2 double-stranded (ds) RNA species encodes toxin and resistance functions in Saccharomyces cerevisiae strains with the K2 killer specificity. RNA sequence analysis reveals the presence of a large open reading frame on the larger heat-cleavage product of M2 dsRNA, which is translated in vitro to yield a 28 kd polypeptide as a major product. The postulated translation initiator AUG triplet is located within a stem and loop structure near the 5' terminus of the positive strand, which also contains plausible 18S and 5.8S ribosomal RNA binding sites. These features may serve to regulate the translation of the K2 toxin precursor. The M1 (from type 1 yeast killers) and M2 dsRNA species lack extensive sequence homology, although specific features are shared, which may represent structural elements required for gene expression and replication.

Documentos Relacionados

• A palindromic mutation of the linear killer plasmid k2 of yeast.
• Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions.
• Influenza A virus M2 ion channel protein: a structure-function analysis.
• Separation and sequence of the 3' termini of M double-stranded RNA from killer yeast.
• Localization of genes for the double-stranded RNA killer virus of yeast.