Structure and formation of circular dimers of simian virus 40 DNA.
AUTOR(ES)
Goff, S P
RESUMO
Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the viral DNA was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. The monomeric units of dimers were organized in a head-to-tail, tandem arrangement; moreover, the monomeric units were not defective; i.e., they lacked deletions or other rearrangements. After infections with dimer DNA, nondefective monomers were formed. These findings suggest that dimers are not intermediates in the production of defective SV40 genomes. The majority of the dimers formed in mixed infections with two mutants were homodimers, but about 5% of the circular dimers were heterodimers and must have arisen by intermolecular recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515930Documentos Relacionados
- Structural state of newly replicated closed circular simian virus 40 DNA.
- Accommodation of pyrimidine dimers during replication of UV-damaged simian virus 40 DNA.
- Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.
- Location of histones on simian virus 40 DNA.
- DNase I sensitivity of integrated simian virus 40 DNA.