Structure and Function of Bacteriophage R17 Replicative Intermediate Ribonucleic Acid II. Properties of the Parental Labeled Molecule
AUTOR(ES)
Erikson, R. L.
RESUMO
Replicative intermediate ribonucleic acid (RNA), designated RI, which contained parental RNA labeled with 32P was separated by filtration through agarose from the nucleic acids prepared from 32P-labeled RNA phage-infected Escherichia coli. A larger amount of ribonuclease-sensitive parental label was found in the rapidly sedimenting forms of RI than in the slower sedimenting forms, indicating that parental RNA is displaced to form a single-stranded tail. This result indicates that some phage RNA is generated by asymmetric semiconservative replication of RI, but it does not mean that a portion of the RI duplexes cannot be conserved during generation of phage RNA. Parental RNA was also found in double-stranded RNA with no apparent tails which sedimented with an S value of 13. This RNA was soluble in 2 m NaCl, and its sedimentation rate was unaffected by ribonuclease; nevertheless, single-strand scissions were produced by ribonuclease and were detected after the duplex was converted to its component single strands.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375271Documentos Relacionados
- Replication of Bacteriophage Ribonucleic Acid: Analysis of the Ultrastructure of the Replicative Form and the Replicative Intermediate of Bacteriophage R17
- Structure of the Ribonucleic Acid Bacteriophage R17
- Denaturation and Renaturation of Viral Ribonucleic Acid II. Characterization of the Products Resulting from Annealing R17 Ribonucleic Acid with Denatured Replicative Form or with Denatured Replicative Intermediate
- Denaturation and Renaturation of Viral Ribonucleic Acid I. Annealing R17 Ribonucleic Acid with Denatured Replicative Form or with Denatured Replicative Intermediate
- Alterations in the Distribution of Labeled Ribonucleic Acid in Polyribosomes from Escherichia coli Infected with Bacteriophage R17