Structure of genes for human growth hormone and chorionic somatomammotropin.
AUTOR(ES)
Fiddes, J C
RESUMO
A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2--3 kb was ligated to lambda gt WES.lambda B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411560Documentos Relacionados
- Immunoreactivity and receptor binding of mixed recombinants of human growth hormone and chorionic somatomammotropin.
- Relative levels of methylation in human growth hormone and chorionic somatomammotropin genes in expressing and non-expressing tissues.
- Human chorionic somatomammotropin and growth hormone gene expression in rat pituitary tumour cells is dependent on proximal promoter sequences.
- Study on dioxan separation in the radioimmunoassay of human growth hormone, human chorionic gonadotrophin, adrenocorticotrophic hormone, and insulin
- STUDIES ON HUMAN GROWTH HORMONE. I. A RADIO-IMMUNOASSAY FOR HUMAN GROWTH HORMONE*
