Structure of the inverted terminal repetition of adenovirus type 2 DNA.
AUTOR(ES)
Wu, M
RESUMO
Several secondary structure features involving the ends of single strands of adenovirus type 2 DNA have been studied by electron microscopy by both the gene 32-ethidium bromide technique and a modification of the standard formamide-cytochrome c technique. A duplex stem of length 115 +/- 10 nucleotide pairs due to pairing between the two members of the inverted terminal repetition is observed in the single-stranded circles that form upon annealing single-stranded linear molecules. This duplex stem is shown to lie at the ends of the DNA by using several reference markers: (i) a newly discovered secondary structure feature (a loop of length ca. 500 nucleotides with a 20-nucleotide pair duplex stem) that maps 73% of the full length from the left end of the molecule and (ii) a duplex region due to a hybridized restriction fragment. There is also some secondary structure within each end of linear single strands. There is some variation in the morphology of the end strucures, and we propose that these involve base pairing, as in a tRNA clover leaf, rather than an exact single hairpin-type inverted repeat. These observations are consistent with the hypothesis that there is a foldback structure at the 3' ends of the DNA that functions as a primer for the initiation of replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353878Documentos Relacionados
- Arrangement of sequences in the inverted terminal repetition of adenovirus 18 DNA.
- Adenovirus-2 DNA Contains an Inverted Terminal Repetition
- Visualization of an inverted terminal repetition in vaccinia virus DNA.
- Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA.
- Site-specific nicking within the adenovirus inverted terminal repetition.
