Studies for the development of new methodology for evaluating quality protein using larvae of Chrysomya chloropyga (WIEDMANN) / Estudos para o desenvolvimento de novo metodo de avaliação de qualidade proteica utilizando larvas de Chrysomya chloropyga (Wiedmann)

AUTOR(ES)
DATA DE PUBLICAÇÃO

1980

RESUMO

The growth of Chrysomya albiceps, Chrysomya chloropyga and Chrysomya megaceplala larvae toward a natural (mice carcass) and milk-yeast diets was studied. The larva of C. chloropyga was selected because of its identical growth in both diets. Experiments were carried out to study the effect of different protein levels and different protein sources as well. Four pratein sources were utilized in this study: l)dried whole milk, 2) dried whole milk-plusyeast, 3) casein and 4) casein cornbined with yeast. The protein levels were 2, 4, 6, 8 and 10% for each one of the diets. In the dried whole milk and dried whole milk-plus-yeast the gain in body weight was proportional to the protein content of the diets, at the levels af 2 and 4%. At 6, 8 and 10% the growth was impaired, probably due to the high solid concentration in the diets. When the caseio and the casein-plus-yeast diets were utilized, the growth was proportional to the protein content of the diets up to the leveI of 8%, in the case of the casein plus-yeast diet, and up to 10% for the casein diet. When yeast was incorporated to the diets, the growth rates were always higher than those obtained without it. This result was not surprising sinee yeasts are a good natural source of ribonucleic acid. The addition of RNA to the casein diet showed even better results, in terms of growth, than the casein-plus-yeast diet. For this reason, the casein diet to which RNA was added, was taken as the standard diet throughout this study. The effect af different protein saurces on the growth rates of C. chloropyga larvas was investigated in another set of experiments. The ability of bean (Phaseolus vulgaris) and soy-bean (Glycine max) proteins to support grawth as well as the effect of heat treatment and L-methionine supplementation were studied. The larvae did not respond to the supplementation of the unheated bean protein with L-methionine, however the growth rates were higher when the flour was heat-treated and when the heated-treated flour was supplemented with L-methionine. In the case of unheated soy flour, there was a better growth response after L-methionine addition; heated soy flour supported higher growth rates than the unheat flour both with and without L-methionine addition. Rice corn and wheat were also testes as protein sources for the C. Chloropyga larvae. Corn and wheat when supplemented with limiting amino acids elicited a better growth response than the unsupplemented sources. Supplementation of corn, rice and wheat with bean and soy-bean protein was beneficial to the growth of C. Chloropyga larvae. Dried whole egg vlas also tested, giving higher growth rates when compared with any other protein source

ASSUNTO(S)

diet proteinas - analise protein inseto - larva insect dietas

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