STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM VII. : Purification and Detoxification of Trypsin-activated Type E Toxin
AUTOR(ES)
Fiock, Mary A.
RESUMO
Fiock, Mary A. (Fort Detrick, Frederick, Md.), Allen Yarinsky, and James T. Duff. Studies on immunity to toxins of Clostridium botulinum. VII. Purification and detoxification of trypsin-activated type E toxin. J. Bacteriol. 82:66–71. 1961.—A procedure was described for production, purification, and conversion to toxoid of the Clostridium botulinum type E trypsin-treated toxin. A medium containing 1.5% trypticase, 0.5% yeast extract, 0.075% cysteine hydrochloride, and 1.0% glucose consistently yielded activated culture toxicities of 300,000 mouse intraperitoneal ld50/ml. Purification of the toxin was accomplished by precipitation with ammonium sulfate, extraction with calcium chloride, and reprecipitation with ethanol in the cold. The resulting partially purified toxin had a specific activity of 45,000,000 ld50/mg nitrogen. Purified toxins were converted to toxoid by incubation with formalin and adsorbed on aluminum phosphate. Good immune responses were obtained to the toxoids in mice, guinea pigs, and rabbits.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279115Documentos Relacionados
- COMPARATIVE GEL FILTRATION OF TOXIN PRECURSOR AND TRYPSIN-ACTIVATED TOXIN OF CLOSTRIDIUM BOTULINUM TYPE E
- STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM III. : Preparation, Purification, and Detoxification of Type E Toxin
- STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM VI. : Purification and Detoxification of Type D Toxin and the Immunological Response to Toxoid
- STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM II. : Production and Purification of Type B Toxin for Toxoid
- STUDIES ON IMMUNITY TO TOXINS OF CLOSTRIDIUM BOTULINUM IV. : Production and Purification of Type C Toxin for Conversion to Toxoid
