Studies on the Rate and Site-Specificity of P Element Transposition

AUTOR(ES)

Berg, C. A.

RESUMO

A single genetically marked P element can be efficiently mobilized to insertionally mutagenize the Drosophila genome. We have investigated how the structure of the starting element and its location along the X chromosome influenced the rate and location of mutations recovered. The structure of two P[rosy(+)] elements strongly affected mobilization by the autonomous ``Jumpstarter-1'' element. Their average transposition rates differed more than 12-fold, while their initial chromosomal location had a smaller effect. The lethal and sterile mutations induced by mobilizing a P[rosy(+)] element from position 1F were compared with those identified previously using a P[neo(R)] element at position 9C. With one possible exception, insertion hotspots for one element were frequently also targets of the other transposon. These experiments suggested that the genomic location of a P element does not usually influence its target sites on nonhomologous chromosomes. During the course of these experiments, Y-linked insertions expressing rosy(+) were recovered, suggesting that marked P elements can sometimes insert and function at heterochromatic sites.

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