Study of the interaction between Gal-1 and mast cell / Estudo da interação de galectina-1 e mastócitos

AUTOR(ES)

Daniel Roberto Callejon

DATA DE PUBLICAÇÃO

2008

RESUMO

Galectin-1 (Gal-1) belongs to a family of ?-galactoside-binding proteins and is involved in several biological processes, including modulation of the inflammatory response. Mast cells play a critical role in allergic and inflammatory events, however, little is known about the impact of Gal-1 on mast cell biology. In this study, we examined the role of Gal-1 in mast cell function using RBL-2H3 (Rat Basophilic Leukemia) and galectin-1 deficient mice. We report that Gal-1 recognized glycoconjugates on mast cells and this interaction promotes phosphatidylserine (PS) exposure in the absence of cell death or apoptosis. Morphological analysis of Gal-1-treated RBL-2H3 cells, by scanning electron microscopy and Differential Interference Contrast (DIC), indicated that Gal-1 induces modifications on cell membranes and modulation of ruffles formation on cell surface of stimulated RBL-2H3 cells. Interesting, Gal-1 treatment of RBL-2H3 cells, with or without stimulation, promotes alterations in the distribution of the components (Lyn, LAT and GD1b) of Lipid Rafts. Gal-1 did not promote degranulation on RBL-2H3 with or without prior sensitization with IgE. However, Gal-1 treatment inhibits the cell degranulation mediated by via Fc?RI and this effect was time and dose-dependent. Also, this inhibition was related to carbohydrate recognition domain and required Gal-1 dimerization. Importantly, confocal microscopy analysis showed that Gal-1 was distributed in cytoplasm close to secretory granules stained AD-1 antibody on RBL-2H3 with or without prior stimulation with Fc?RI. In addition, we found significantly increased passive cutaneous anaphylaxis reaction in Gal-1 deficient mice. The results demonstrated that Gal-1 may participate of homeostasis and exocytose in mast cells suggesting that Gal-1 can have important role in allergic and inflammatory process.

ASSUNTO(S)

apoptosis degranulation galectina-1 mastócito mast cell desgranulação apoptose inflamação galectin-1 inflammation

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