Study of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases.

AUTOR(ES)

Bonnet, J

RESUMO

Several studies have clearly demonstrated that the end of the acceptor stem was a very important area determining the aminoacylation properties of tRNAs. However the attempts to measure the contribution of this region to the binding of tRNAs to aminoacyl-tRNA synthetases have led to contradictory results. We report here the stepwise degradation of yeast tRNA-Phe and tRNA-Val from their 3' terminus, up to the seventh nucleotide : the affinity of each of the degraded-tRNA for their cognate aminoacyl-tRNA synthetase was compared to that of intact tRNA and it was found that these affinities are not significantly decreased when compared to those of the intact tRNAs.

Documentos Relacionados

• The N49-N65 base-pair could play an important role in adaptation between tRNAs and aminoacyl-tRNA synthetases.
• Retrovirus-Specific Packaging of Aminoacyl-tRNA Synthetases with Cognate Primer tRNAs
• Identification and Origin of Some Chloroplast Aminoacyl-tRNA Synthetases and tRNAs*
• Translocation events in the evolution of aminoacyl-tRNA synthetases.
• Sequence, structural and evolutionary relationships between class 2 aminoacyl-tRNA synthetases.