Subcellular localization of the simian virus 40 agnoprotein.

AUTOR(ES)

Nomura, S

RESUMO

The intracellular distribution of the simian virus 40 agnoprotein in infected cells was determined by indirect immunofluorescence and biochemical fractionation followed by indirect immunoprecipitation. The specific antibodies used in these studies were directed against either purified agnoprotein or a synthetic oligopeptide homologous to the N-terminus of the processed protein. Both procedures showed predominant localization of the agnoprotein to the cytosol and to the perinuclear region in association with the outer nuclear membrane. A minor but detectable fraction of the protein was also found in the nucleus. The definition of its subcellular distribution, as well as its high lability in vivo and affinity for nucleic acid, provide a basis for speculation on the function of this gene product.

Documentos Relacionados

• The growth of simian virus 40 (SV40) host range/adenovirus helper function mutants in an African green monkey cell line that constitutively expresses the SV40 agnoprotein.
• Subcellular Localization of Simian Virus 40 Large Tumor Antigen
• Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein.
• Role of the agnoprotein in regulation of simian virus 40 replication and maturation pathways.
• Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus.