Sugarcane juice supplementation compared to others carbohydrates on glycogen stores and recovery kinetics of biomarkers after a bout of acute exercise in rats / Suplementação com caldo de cana de açucar comparado com outros carboidratos na reposição do glicogenio e cinetica de biomarcadores pos-exercicio agudo em ratos

Eduarda Faria Abrahão Machado

Physical exercise induces traumas to biological structures which signal inflammatory process activation. The repair and regeneration of the damaged structures depend on an appropriate rest period for the recovery. This regenerative period is necessary after a single session or after several sessions of exercises. The muscle glycogen repletion after physical exercise seems to influence the recovery time. Usually in practices the carbohydrate rich compounds like maltodextrine, fructose or dextrin are normally used to maximize the rate of glycogen storage in the early hour s post-exercise. Sugar cane juice is an appreciate product and easily to find in Brazil. It possesses 65%-75% of water and a high sucrose concentration that corresponds 70%-91% of their soluble solids, phenolics compounds, vitamins, minerals and amino acids. The goal of this master s degree dissertation was to analyze the effect of sugar cane juice comparatively to other carbohydrates solutions supplied immediately after a bout of exhausting exercise in rats in the replacement of the glycogen stores; and in the kinetics of some biomarkers in 48h post-effort. However, the literature revision found the use of several anesthetics as the main methodological variation in the studies that quantified glycogen concentration in animals. It was necessary, therefore, initially to define the anesthetic that could allow the association of the data obtained in tissues with concomitant analyses in blood. It was important to know if the anesthetic used for samples collection could cause haemolysis and/or glycogenolysis in the animals. The studies accomplished during the master s degree are contained in two chapters. In chapter 1 we presented the comparison data of three injectable anesthetics used in experiments with animals, concerning the degree of haemolysis and glycogenolysis after anesthesia. The animals were divided into three groups: Cloral Hydrate (CH), Ketamine + Xylazine (KX), Zoletil 50® (zolazepam and tiletamine) + Xylazine (ZTX). The CH and KX presented serum haemolysis. Only ZTX presents no detectable values. The average value of the hepatic and muscular glycogen concentrations exhibited no significant difference between CH and ZTX. However, the KX presented accentuated glycogenolysis in all tissues. Our data suggest that the anesthetic ZTX seems to be the most appropriate for studies that need simultaneously to quantify the concentration of glycogen and blood markers without interferences. Chapter 2 presented the data of the effect of sugar cane juice comparatively to other carbohydrates solutions supplied after the exhaustion in rats, in the replacement of muscular and hepatic glycogen stores, and in the kinetics of some markers of proteolyses, muscular lesion and inflammation in 48hs of recovery. For that, we divided this study in two experiments. The results presented in the experiment 1 showed that the supplementation with sugar cane was as efficient as maltodextrine to restore the gastrocnemium red and white portions glycogen after 1 h of the exhaustion. The fructose exhibited less pronounced effect. None of the supplements were able to restore the hepatic glycogen significantly. The biochemical parameters and WBC number data analyzed in experiment 2 showed an persistent inflammatory picture associated to muscular damage even after 48h of rest. The supplemented groups with sugar cane and maltodextrine didn t alter the 48h post-exercise response pattern. Their representatives potentialities reinforce the continuity of the investigations

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