Synchronization of cell division in microorganisms by percoll gradients.
AUTOR(ES)
Dwek, R D
RESUMO
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=294577Documentos Relacionados
- Variation in Escherichia coli buoyant density measured in Percoll gradients.
- SYNCHRONIZATION OF CELL DIVISION
- Freeze-cutting of density gradients.
- Synchronization of Cell Division in Escherichia coli by Elevated Temperatures: a Reinterpretation
- Isolation of newly replicated chromatin by using shallow metrizamide gradients.