Syncytium-induction inhibition test with complement for detection of antibodies against bovine leukemia virus.
AUTOR(ES)
Kono, Y
RESUMO
A modified syncytium-induction inhibition test which is more sensitive than the immunodiffusion test, was developed using rabbit complement. In this test, fetal lamb kidney cells continuously infected with bovine leukemia virus were used as effector cells, and the CC81 cat cells transformed with murine sarcoma virus, were used as indicator cells. The syncytium-induction inhibition effect of anti-bovine leukemia virus serum was enhanced significantly by the addition of rabbit complement. The syncytium-induction inhibition titers had a statistically significant correlation with the immunodiffusion titers and were four to 64 times higher than immunodiffusion titers. In 12 experimentally infected cattle, the syncytium-induction inhibition test detected the antibodies earlier than the immunodiffusion test and continuously detected them when immunodiffusion antibody changed to negative. In the 81 sera from naturally infected herds, 35 (43.2%) were positive by the immunodiffusion test and 55 (67.9%) by the syncytium-induction inhibition test.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1235947Documentos Relacionados
- Proteins of bovine leukemia virus. I. Characterization and reactions with natural antibodies.
- Comparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus.
- Microtiter Hemagglutination-Inhibition Test for the Detection of Encephalomyocarditis Virus Antibodies
- Early Syncytium Formation by Bovine Leukemia Virus
- Inhibition of protein kinase C results in decreased expression of bovine leukemia virus.
