Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
AUTOR(ES)
Hoffmann, P U
RESUMO
The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be more efficient when the adenylated form of the donor molecule is used.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=305962Documentos Relacionados
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