Synthesis and Secretion of Gammaglobulin by Lymph Node Cells: The Acquisition of Carbohydrate Residues of Immunoglobulin in Relation to Interchain Disulfide Bond Formation*

AUTOR(ES)

Sutherland, Earl W.

RESUMO

Acquisition of carbohydrates in the disulfide-linked heavy (H) and light (L) chain molecules of murine myeloma (ADJPC5), i.e., HH, HHL, and LHHL, was investigated. That some mannose and glucosamine residues are acquired by immunoglobulin precursor molecules was demonstrated by the detection of glucosamine and mannose in HH, HHL, and LHHL. In contrast, galactose was observed solely in LHHL molecules, which have an identical electrophoretic mobility to the secreted product. Furthermore, as judged from cells incubated with [3H]leucine, the more juvenile molecules HH and HHL were predominant in the rough microsome fraction, whereas LHHL was the principal molecular species in the smooth microsome fraction.

Documentos Relacionados

• The synthesis and secretion of gamma-globulin by lymph node cells. 3. The slow acquisition of the carbohydrate moiety of gamma-globulin and its relationship to secretion.
• Synthesis and Secretion of γ-Globulin by Lymph Node Cells, VIII. Order of Synthesis of the Interchain Disulfide Linkages of Immunoglobulins*
• Opposite Effects of BCG on Spleen and Lymph Node Cells: Lymphocyte Proliferation and Immunoglobulin Synthesis
• THE SYNTHESIS AND SECRETION OF γ-GLOBULINS BY LYMPH NODE CELLS, I. THE MICROSOMAL COMPARTMENTALIZATION OF γ-GLOBULINS
• Redox Regulation of the Human Dual Specificity Phosphatase YVH1 through Disulfide Bond Formation*