Synthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domain.

AUTOR(ES)

Amin, A A

RESUMO

We have used an in vitro transcription and translation system to synthesize an enzymatically active FLP protein. The FLP mRNA synthesized in vitro by SP6 polymerase is translated efficiently in a rabbit reticulocyte lysate to produce enzymatically active FLP. Using this system, we assessed the effect of deletions and tetrapeptide insertions on the ability of the respective variant proteins synthesized in vitro to bind to the FLP recognition target site and to carry out excisive recombination. Deletions of as few as six amino acids from either the carboxy- or amino-terminal region of FLP resulted in loss of binding activity. Likewise, insertions at amino acid positions 79, 203, and 286 abolished DNA-binding activity. On the other hand, a protein with an insertion at amino acid 364 retained significant DNA-binding activity but had no detectable recombination activity. Also, an insertion at amino acid 115 had no measurable effect on DNA binding, but recombination was reduced by 95%. In addition, an insertion at amino acid 411 had no effect on DNA binding and recombination. On the basis of these results, we conclude that this approach fails to define a discrete DNA-binding domain. The possible reasons for this result are discussed.

Documentos Relacionados

• RNP-1, an RNA-binding motif is conserved in the DNA-binding cold shock domain.
• TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain.
• Structural domains in phage Mu transposase: identification of the site-specific DNA-binding domain.
• Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain.
• A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain.