Synthesis of ppTppp via phosphotriester intermediates.
AUTOR(ES)
Schattenkerk, C
RESUMO
The bifunctional and crystalline phosphorylating agent morpholino-0,0-bis[1-benzotriazolyl]phosphate has been used for the preparation of a 3',5'-bis-phosphotriester intermediate of thymidine. The latter has been converted into ppTppp by the following consecutive steps; removal of the benzotriazolyl group followed by the addition of phosphoric acid and removal of the 2-(4-nitrophenyl)-ethyl group followed by the addition of pyrophosphoric acid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=326501Documentos Relacionados
- Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediates.
- New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.
- A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
- Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
- Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
