Synthesis of RNA containing a methylated blocked 5′ terminus by HeLa nuclear homogenates

AUTOR(ES)

Groner, Yoram

RESUMO

RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5′-terminal structures that can be grouped into four general types: (a) m7GppNmp...; (b) m7GppNmpMmp...; (c) m7GpppNmp...; and (d) m7GpppNmpMm.... Both Nm and Mm can be either one of the four 2′-O-methylated nucleosides. The relative amounts of the Nm species are G = 45%, A = 25%, U = 20%, and C = 10%.

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