Syringe method for stepwise chemical synthesis of oligonucleotides.
AUTOR(ES)
Tanaka, T
RESUMO
A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in isolation procedures are also presented.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320704Documentos Relacionados
- The case of sulfonation in the chemical synthesis of oligonucleotides.
- Stepwise synthesis of oligonucleotides. XXII. The synthesis of Tpsi-loop fragments of yeast tRNAIVal and their analogs.
- A general method for the synthesis of 3'-sulfhydryl and phosphate group containing oligonucleotides.
- Enzymatic synthesis of RNA oligonucleotides.
- Synthesis and application of derivatizable oligonucleotides.