Systematic investigation of protein phase behavior with a microfluidic formulator
AUTOR(ES)
Hansen, Carl L.
FONTE
National Academy of Sciences
RESUMO
We demonstrated a microfluidic device for rapidly generating complex mixtures of 32 stock reagents in a 5-nl reactor. This “formulation chip” is fully automated and allows thousands of experiments to be performed in a single day with minimal reagent consumption. It was applied to systematically study the phase behavior of the protein xylanase over a large and complex chemical space. For each chemical formulation that demonstrated a pronounced effect on solubility, the protein phase behavior was completely mapped in the chip, generating a set of empirical phase diagrams. This ab initio phase information was used to devise a rational crystallization screen that resulted in 72-fold improvement in successful crystallization hits compared with conventional sparse matrix screens. This formulations tool allows a physics-based approach to protein crystallization that may prove useful in structural genomics efforts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=521965Documentos Relacionados
- An investigation of the behavior of a classifying hydrocyclone with pseudoplastic fluids
- Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase.
- Microfluidic tectonics: A comprehensive construction platform for microfluidic systems
- Mixed states and suicidal behavior: a systematic review
- Sedentary behavior among university students: a systematic review
