Targeting of proteins to the outer envelope membrane uses a different pathway than transport into chloroplasts.
AUTOR(ES)
Li, H M
RESUMO
The chloroplastic envelope is composed of two membranes, inner and outer, each with a distinct set of polypeptides. Like proteins in other chloroplastic compartments, most envelope proteins are synthesized in the cytosol and post-translationally imported into chloroplasts. Considerable knowledge has been obtained concerning protein import proteins. We isolated a cDNA clone from pea that encodes a 14-kilodalton outer envelope membrane protein. The precursor form of this protein does not possess a cleavable transit peptide and its import into isolated chloroplasts does not require either ATP or a thermolysin-sensitive component on the chloroplastic surface. These findings, together with similar observations made with a spinach chloroplastic outer membrane protein, led us to propose that proteins destined for the outer membrane of the chloroplastic envelope follow an import pathway distinct from that followed by proteins destined for other chloroplastic compartments.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=160038Documentos Relacionados
- An expression cassette for targeting foreign proteins into chloroplasts.
- Insertion of OEP14 into the Outer Envelope Membrane Is Mediated by Proteinaceous Components of Chloroplasts
- A GTP-driven motor moves proteins across the outer envelope of chloroplasts
- Protein targeting and integration signal for the chloroplastic outer envelope membrane.
- Insertion of a MalE β-Galactosidase Fusion Protein into the Envelope of Escherichia coli Disrupts Biogenesis of Outer Membrane Proteins and Processing of Inner Membrane Proteins