Taxonomia das espécies sul-brasileiras de Calydorea Herbert (Iridaceae) e caracterização por DNA "Barcode"
AUTOR(ES)
Leandro Dal Ri
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
2012
RESUMO
This study aimed a taxonomic revision of the genus Calydorea from southern Brazil, providing the basis for identification of the species, by means of a dichotomous key, illustrations and images of plants in the natural environment. Another aim was to verify the effectiveness of use of target gene sequences to discriminate species, in the light of the proposed DNA "barcode", through the measurement of intra and interspecific variability and the interpretation of the trees obtained from cluster analysis. Collection of plants in field trips through Southern Brazil were performed, and exsiccates from Brazilian herbaria and from foreign countries were analyzed. The results of the taxonomic work indicated five species of the genus Calydorea occurring in Southern Brazil: C. alba Roitman &A. Castillo, C. approximata R.C. Foster, C. basaltica Ravenna, C. campestris (Klatt) Baker and C. crocoides Ravenna. Other species mentioned for the region are also presented and discussed. It was made a lectotypification in Calydorea basaltica and one sinonymization in this taxon. The study of the applicability of the proposed sequences for the DNA "barcode" focused on the variability of five sections of the plastid genome (matK, rbcL, rps4, rps16 and trnL-F) in different accessions of 14 species of Calydorea and two species of Cardiostigma. Cluster analysis with Neighbor-Joining method demonstrated that the power of discrimination of matK gene was the highest, followed by rps16. Four groups of species were evident in all topologies, with small variations and the groups formed are related to morphological traits of the species, saved few exceptions. Phylogenetic inferences can be visualized and correlated with previously published observations. The use of DNA markers "barcode" in Calydorea is promising, although the increase of molecular data from different accessions is needed to determine intra and interspecific variability with more precision.
ASSUNTO(S)
taxonomia vegetal : iridaceae : brasil : rio grande do sul : teses regiao sul
ACESSO AO ARTIGO
http://hdl.handle.net/10183/55909Documentos Relacionados
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