Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium.

AUTOR(ES)

Dendinger, S M

RESUMO

Mutants of Salmonella typhimurium defective in glutamate dehydrogenase activity were isolated in parent strains lacking glutamate synthase activity by localizcd mutagenesis or by a general mutagenesis combined with a cycloserine enrichment for glutamate auxotrophs. Two mutants with temperature-sensitive phenotypes had glutamate dehydrogenase activities that were more thermolabile than that of an isogenic control strain. Eight other mutants had less than 10% of the wild-type glutamate dehydrogenase activity. All the mutations were cotransducible with a Tn10 element (zed-2:Tn10) located at approximately 23 U on the S. typhimurium linkage map. These data strongly indicate that this region contains the structural gene (gdhA) for glutamate dehydrogenase.

Documentos Relacionados

• Genetic Analysis of Temperature-Sensitive Lethal Mutants of Salmonella Typhimurium
• Flagellar assembly in Salmonella typhimurium: analysis with temperature-sensitive mutants.
• Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase.
• Isolation of temperature-sensitive pantothenate kinase mutants of Salmonella typhimurium and mapping of the coaA gene.
• Temperature-sensitive mutants of Neisseria gonorrhoeae.