Temperature-Sensitive Mutants Isolated from L Cells Persistently Infected with Newcastle Disease Virus
AUTOR(ES)
Preble, Olivia T.
RESUMO
Virus mutants (NDVpi) isolated from L cells persistently infected with the Herts strain of Newcastle disease virus have been previously reported by this laboratory to differ from the wild-type virus (NDVo) in several physical and biological properties. It has now been determined that, in addition to these differences, the NDVpi mutants are also spontaneously selected temperature-sensitive mutants. The temperature sensitivity of 10 NDVpi clones was confirmed by temperature inhibition, plaquing efficiency, and single-cycle yield experiments. The cut-off temperature, at which more than 90% of virus replication is inhibited was between 41 and 42 C. All 10 NDVpi clones were also found to be defective in virus-specific ribonucleic acid (RNA) synthesis in infected chick embryo cells at 42 C and are tentatively classified as RNA−. The possible relationships of the temperature sensitivity, the other NDVpi properties, and the maintenance of the persistently infected state are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356283Documentos Relacionados
- Temperature-Sensitive Defect of Mutants Isolated from L Cells Persistently Infected with Newcastle Disease Virus
- Temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with Newcastle disease virus.
- Comparison of RNA Polymerase Associated With Newcastle Disease Virus and a Temperature-Sensitive Mutant of Newcastle Disease Virus Isolated from Persistently Infected L Cells
- Cells Persistently Infected with Newcastle Disease Virus: I. Properties of Mutants Isolated from Persistently Infected L Cells
- Temperature-sensitive virus derived from BHK cells persistently infected with HVJ (Sendai virus).