Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.

AUTOR(ES)

Martindale, D W

RESUMO

The physical organization of eight Tetrahymena genes active during conjugation (meiosis) was examined in the somatic (macro-) and germinal (micro-) nuclei of this organism. Three of these genes make transcripts that are only detected during meiotic prophase. Southern blot analyses indicated that all genes examined were present in both nuclei. Except for one gene family (pC6), all appeared to be non-repetitive and there were no detectable sequence rearrangements in or near the genes. The exceptional gene was repeated approximately five to seven times and DNA rearrangement occurred in or near each of these copies. A comparison of cDNA and macro- and micronuclear DNA restriction maps indicated that one of the genes (cnj B) contains introns. This is the first report of evidence for introns in a non-ribosomal gene in Tetrahymena. A site specific modification probably due to adenine methylation was seen in the macronuclear copy of another gene (cnj C).

Documentos Relacionados

• Tetrahymena H4 genes: structure, evolution and organization in macro- and micronuclei.
• Conjugation-induced lysis of Schizosaccharomyces pombe.
• Pipeline for macro- and microarray analyses
• Scanning electron microscopy of Neisseria gonorrhoeae. Age-induced changes in macro- and microstructure of virulent and avirulent colonies.
• Macro- and microscopic properties of milk proteins and k-carrageenan gels