The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.
AUTOR(ES)
Klemenz, R
RESUMO
The 5' terminus of Saccharomyces cereviasiae 35S pre rRNA was mapped on the rDNA using two methods: 1) Suitable restriction endonuclease fragments were hybridized to total high molecular weight RNA and extended with reverse transcriptase to the 5' end of the RNA template. 2) Other restriction fragments spanning the 5' terminus of 35S pre rRNA and radioactively labeled at their ends were hybridized to high molecular weight RNA and the non hybridized nucleic acids were digested with S1 nuclease. On the basis of these experiments, the 5' terminus of 35S pre rRNA was placed approximately 670 nucleotides upstream from the 17S rRNA coding region. The exact position was determined by reverse transcription as above, but in the presence of dideoxyribonucleoside triphosphates, which served as a way of sequencing the 5' terminal region. 35S pre rRNA synthesis is initiated at a site in EcoRI restriction fragment B which is 48 nucleotides upstream from the EcoRI cleavage site in the coding strand.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324113Documentos Relacionados
- A putative precursor for the small ribosomal RNA from mitochondria of Saccharomyces cerevisiae.
- Tandemly arranged variant 5S ribosomal RNA genes in the yeast Saccharomyces cerevisiae.
- Magnification of genes coding for ribosomal RNA in Saccharomyces cerevisiae.
- Escherichia coli 23S ribosomal RNA truncated at its 5' terminus.
- Sequence of the region 5' to the negative regulatory gene PHO80 of Saccharomyces cerevisiae.
