The analysis of the human hemopexin promoter defines a new class of liver-specific genes.
AUTOR(ES)
Poli, V
RESUMO
Hemopexin (Hpx) is a plasma glycoprotein which is expressed only in the liver. It is synthesized at a lower rate in the fetal liver than in the adult, and its level increases during acute infections. As shown here, a fragment of the human hemopexin promoter spanning from positions -130 to +22 relative to the cap site is sufficient to direct cell-specific transcription of a reporter gene. Within this segment a short sequence, located between positions -120 and -104, is responsible for this effect. This positive cis-acting element, the Hpx A site, interacts with a family of nuclear proteins, some of which are present only in hepatoma cells. The potential meaning of these complex DNA-protein interactions and the homology with elements present on the promoter of other liver-specific and acute phase genes are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=335137Documentos Relacionados
- Two distinct factors interact with the promoter regions of several liver-specific genes.
- Interleukin 6 induces a liver-specific nuclear protein that binds to the promoter of acute-phase genes.
- Liver-specific gene expression: A-activator-binding site, a promoter module present in vitellogenin and acute-phase genes.
- The transcription factor LF-A1 interacts with a bipartite recognition sequence in the promoter regions of several liver-specific genes.
- Analysis by cell-free transcription of the liver-specific pyruvate kinase gene promoter.