THE APPLICATION OF MESENCHYMAL STEM CELLS CULTURED IN THE PRESENCE OF FGF-2 FOR THE REPAIR OF OSSEOUS DEFECTS IN RATS / APLICAÇÃO DE CÉLULAS-TRONCO MESENQUIMAIS CULTIVADAS COM FGF-2 NA REGENERAÇÀO ÓSSEA DE RATOS

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

There is considerable interest in the therapeutic use of mesenchymal stem cell (MSC) implantation to repair osseous defects. A basic requirement for the application of such strategy is the obtention of MSCs in sufficient numbers, which may be directly influenced by the culture conditions used for expansion of the population. Although there are a number of studies showing that MSCs expanded in media with fibroblast growth factor -2 (FGF-2) have greater osteogenic capacity in vitro, it is not clear whether the implantation of these cells into bone defects will result in a more efficient repair. The aim of this in vivo study was to evaluate histologically the repair of osseous defects following the implantation of MSCs cultured in medium supplemented and not supplemented with FGF-2. Bone marrow mesenchymal stem cells were initially cultured in B-MEM containing (TG1) or not containing FGF-2 (TG2) during 14 days. Cell suspensions of both groups were carried onto collagen sponges and implanted into surgically created osseous defects on the tibia of 12 Wistar isogeneic rats. Some defects received only the collagen sponge and served as controls (CN). Cells of both groups were also investigated for the osteogenic capacity in vitro using a bone-nodule forming assay. At day 7 in vitro, intense cell proliferation was evidenced with bone nodule formation being observed between days 14 and 21. The histological analysis revealed that at day 7 in vivo bone formation was greater in TG1 than in TG2 and CN. However, at day 14 the amount of bone evidenced was similar in TG1 and TG2 although both superior to the control. At day 21, bone formation was clearly superior in TG1 and TG2 than in CN. Moreover, TG1 containing defects showed a more mature and organized bone when compared to the other groups. These results suggest both, that the MSCs have osteogenic potential in vitro and that the implantation of MSCs cultured in medium containing FGF-2 may in vivo initiate more rapidly and in a more organized fashion the repair process.

ASSUNTO(S)

células-tronco stem cells regeneração Óssea fibroblastic growth factor odontologia fator de crescimento fibroblástico tissue engineering engenharia tecidual bone regeneration

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