THE BIOSYNTHESIS OF FERREDOXIN IN A CELL-FREE SYSTEM*
AUTOR(ES)
Trakatellis, Anthony C.
RESUMO
The investigation reported in this communication was concerned with the biosynthesis of ferredoxin in a cell-free system. A cell-free system, with the ability to incorporate amino acids into peptides and proteins, was developed from Cl. pasteurianum, and its requirements were established. After the incorporation of L-alanine-C14, ferredoxin-C14 was isolated in crystalline form with the aid of added carrier ferredoxin. Amino acid analyses and absorption spectra strongly indicated that the isolated ferredoxin-C14, crystallized three times, was essentially free from impurities. Furthermore, experiments with Fe59 strongly indicated that the iron may combine with the apoprotein after the latter is completely synthesized and released from the polysomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=223583Documentos Relacionados
- PROTEIN BIOSYNTHESIS BY A CELL-FREE BACTERIAL SYSTEM*
- A FACTOR PROMOTING THE INITIATION OF GLOBIN SYNTHESIS IN A RABBIT RETICULOCYTE CELL-FREE SYSTEM*
- Biosynthesis of T1 Antigen in Salmonella: Biosynthesis in a Cell-Free System
- DNA-DIRECTED PEPTIDE SYNTHESIS, VI. REGULATING THE EXPRESSION OF THE Lac OPERON IN A CELL-FREE SYSTEM*
- Protochlorophyll Biosynthesis in a Cell-free System from Higher Plants 1
