The Bvg Virulence Control System Regulates Biofilm Formation in Bordetella bronchiseptica
AUTOR(ES)
Irie, Yasuhiko
FONTE
American Society for Microbiology
RESUMO
Bordetella species utilize the BvgAS (Bordetella virulence gene) two-component signal transduction system to sense the environment and regulate gene expression among at least three phases: a virulent Bvg+ phase, a nonvirulent Bvg− phase, and an intermediate Bvgi phase. Genes expressed in the Bvg+ phase encode known virulence factors, including adhesins such as filamentous hemagglutinin (FHA) and fimbriae, as well as toxins such as the bifunctional adenylate cyclase/hemolysin (ACY). Previous studies showed that in the Bvgi phase, FHA and fimbriae continue to be expressed, but ACY expression is significantly downregulated. In this report, we determine that Bordetella bronchiseptica can form biofilms in vitro and that the generation of biofilm is maximal in the Bvgi phase. We show that FHA is required for maximal biofilm formation and that fimbriae may also contribute to this phenotype. However, expression of ACY inhibits biofilm formation, most likely via interactions with FHA. Therefore, the coordinated regulation of adhesins and ACY expression leads to maximal biofilm formation in the Bvgi phase in B. bronchiseptica.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=516841Documentos Relacionados
- Flagellin gene transcription in Bordetella bronchiseptica is regulated by the BvgAS virulence control system.
- The BvgAS Signal Transduction System Regulates Biofilm Development in Bordetella
- Alcaligin Siderophore Production by Bordetella bronchiseptica Strain RB50 Is Not Repressed by the BvgAS Virulence Control System
- Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells
- The bvgAS locus negatively controls motility and synthesis of flagella in Bordetella bronchiseptica.