The chorionic gonadotropin alpha-subunit gene is on human chromosome 18 in JEG cells.
AUTOR(ES)
Hardin, J W
RESUMO
The gene for the alpha subunit of human chorionic gonadotropin (hCG) has been tentatively assigned to human chromosome 18. This localization was accomplished through the use of Southern blot analysis. A full-length cDNA probe for the hCG alpha subunit and DNA isolated from a series of somatic hybrids between mouse and human cells were utilized to make this assignment. In addition, in situ hybridization with normal human peripheral blood lymphocytes as a source of human chromosomes and with the same cDNA probe confirmed this result. The presence of human chromosome 18 was required for the detection of DNA fragments characteristic of the alpha-hCG gene. These results are consistent with our previous observation that human chromosomes 10 and 18 are required for the production of hCG in cultured cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=394280Documentos Relacionados
- GATA-binding proteins regulate the human gonadotropin alpha-subunit gene in the placenta and pituitary gland.
- Characterization of the human rod transducin alpha-subunit gene.
- Cyclic AMP regulation of the human glycoprotein hormone alpha-subunit gene is mediated by an 18-base-pair element.
- G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.
- The pea mitochondrial ATPase subunit 9 gene is located upstream of the ATPase alpha-subunit gene.