The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.

AUTOR(ES)

Houghton, M

RESUMO

Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.

Documentos Relacionados

• The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.
• NH2-terminal amino acid sequence of human fibroblast interferon.
• Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
• Domain structure of the human immunodeficiency virus reverse transcriptase.
• PPi analogs as inhibitors of human T-lymphotropic virus type III reverse transcriptase.