The Comprehensive Mouse Radiation Hybrid Map Densely Cross-Referenced to the Recombination Map: A Tool to Support the Sequence Assemblies

AUTOR(ES)

Rowe, Lucy B.

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

We have developed a unique comprehensive mouse radiation hybrid (RH) map of nearly 23,000 markers integrating data from three international genome centers and over 400 independent laboratories. We have cross-referenced this map to the 0.5-cM resolution recombination-based Jackson Laboratory (TJL) backcross panel map, building a complete set of RH framework chromosome maps based on a high density of known-ordered anchor markers. We have systematically typed markers to improve coverage and resolve discrepancies, and have reanalyzed data sets as needed. The cross-linking of the RH and recombination maps has resulted in a highly accurate genome-wide map with consistent marker order. We have compared these linked framework maps to the Ensembl mouse genome sequence assembly, and show that they are a useful medium resolution tool for both validating sequence assembly and elucidating chromosome biology.

Documentos Relacionados

• A First-Generation Whole Genome–Radiation Hybrid Map Spanning the Mouse Genome
• Construction of a radiation hybrid map of chicken chromosome 2 and alignment to the chicken draft sequence
• A Comprehensive Transcript Map of the Mouse Gnas Imprinted Complex
• A 1-Mb resolution radiation hybrid map of the canine genome
• A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping