The Cys607→Tyr Change in the UL97 Phosphotransferase Confers Ganciclovir Resistance to Two Human Cytomegalovirus Strains Recovered from Two Immunocompromised Patients

AUTOR(ES)

Baldanti, Fausto

FONTE

American Society for Microbiology

RESUMO

Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607→Tyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.

Documentos Relacionados

• A Nine-Codon Deletion Mutation in the Cytomegalovirus UL97 Phosphotransferase Gene Confers Resistance to Ganciclovir
• Human cytomegalovirus UL97 kinase confers ganciclovir susceptibility to recombinant vaccinia virus.
• Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir.
• Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
• Mutations in human cytomegalovirus UL97 gene confer clinical resistance to ganciclovir and can be detected directly in patient plasma.