The effect of protein–precipitant interfaces and applied shear on the nucleation and growth of lysozyme crystals

AUTOR(ES)
FONTE

International Union of Crystallography

RESUMO

The nucleation of lysozyme in microbatch experiments was linked to the formation of protein–precipitant interfaces. The use of oscillatory shear allowed decreasing the nucleation rate and extending the growth period for lysozyme crystals, presumably through the control of the number of interfaces and removal of impurities or defects.

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