The effects of atropine on [3H]acetylcholine secretion from guinea-pig myenteric plexus evoked electrically or by high potassium

AUTOR(ES)

Alberts, P.

RESUMO

1. The myenteric plexus-longitudinal muscle preparation of the guinea-pig ileum was incubated with [3H]choline (1·125-1·5 μM), and then superfused with Tyrode solution containing hemicholinium-3 (10 μM). Secretion of [3H]acetylcholine ([3H]ACh) was evoked either (a) by electrical field stimulation (0·5-15 Hz, 150 shocks per period, 0·5 msec), used to `indirectly' depolarize the varicosities of nerve terminals, or (b) by high potassium (40 mM with 1 μM-tetrodotoxin, for 6 min, or 80 mM without tetrodotoxin, for 1 min), to `directly' depolarize varicosities.

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