The enhanced peroxidase one step method increases sensitivity for detection of Ki-67 in pituitary tumours.
AUTOR(ES)
Turner, H E
RESUMO
AIM: To compare the Ki-67 labelling index determined using the enhanced peroxidase one step (EPOS) method with that using the standard ABC technique in pituitary tumours. METHODS: Adjacent sections were immunostained using the EPOS and ABC techniques with the same Ki-67 antibody and same antigen retrieval method. RESULTS: The labelling index measurements with the EPOS Ki-67 antibody were significantly higher than when using the traditional ABC method, and there was a positive correlation between the two techniques when performed on the same cases. This suggests that a higher proportion of cells are within the cell cycle than previously thought, although it gives no direct information on the rate of proliferation of the tumour. CONCLUSIONS: It appears that the EPOS system is not only more convenient but may be more sensitive than traditional techniques for detecting Ki-67 in the nuclei of cells, thus demonstrating more accurately which cells have entered the cell cycle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=500957Documentos Relacionados
- Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody
- Lack of correlation between Ki-67 labelling index and tumor size of anterior pituitary adenomas
- Apoplexy in small pituitary tumours.
- Prognostic indicators in a range of astrocytic tumours: an immunohistochemical study with Ki-67 and p53 antibodies.
- Ki-67 expression in anal intraepithelial neoplasia in AIDS