The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli.
AUTOR(ES)
Cox, M M
RESUMO
The FLP gene of the yeast 2-microns plasmid is involved in a site-specific recombination event that results in the inversion of a set of sequences within the plasmid. This gene has been cloned and expressed in Escherichia coli. Expression of the FLP gene results in efficient recombination within the bacterial cell, which is specific for plasmids containing at least one 2-microns plasmid recombination site. This work demonstrates that (i) FLP protein is actively involved in 2-microns plasmid recombination; (ii) no other factors specific to yeast are required for the reaction; (iii) FLP protein acts efficiently in trans; (iv) FLP protein will promote site-specific insertion and deletion reactions in addition to the inversion reaction; and (v) FLP-promoted recombination is not dependent upon any DNA structural features unique to yeast chromatin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=384009Documentos Relacionados
- The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site.
- Nucleosome organization of the yeast 2-micrometer DNA plasmid: a eukaryotic minichromosome.
- Yeast 2-microns plasmid DNA replication in vitro: purification of the CDC8 gene product by complementation assay.
- Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle.
- Functional genetic expression of eukaryotic DNA in Escherichia coli.
