The Gene Structures of Spontaneous Mutations Affecting a CAENORHABDITIS ELEGANS Myosin Heavy Chain Gene
AUTOR(ES)
Eide, David
RESUMO
We have isolated spontaneous mutations affecting the unc-54 major myosin heavy chain gene of Caenorhabditis elegans (variety Bristol). Spontaneous unc-54 mutants occur in C. elegans populations at a frequency of approximately 3 x 10-7. We have studied the gene structure of 65 independent unc-54 mutations using filter-transfer hybridization techniques. Most unc-54 mutations (50 of 65) exhibit no abnormalities detected with these techniques; these mutations are small lesions affecting less than 100 base pairs. Approximately 17% of the mutations (11 of 65) are simple deletions, ranging in size from less than 100 base pairs to greater than 17 kilobases. One isolate contains two separate deletions, each of which affects unc-54. Two mutants contain tandem genetic duplications that include a portion of unc-54 and extend 8–10 kilobases beyond the 5' terminus of the mRNA. Conspicuously absent from our collection of spontaneous unc-54 mutations are any resulting from insertion of transposable genetic elements. Such mutants, if they occur, must arise at a frequency of less than 5 x 10-9
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1202484Documentos Relacionados
- A selection for myosin heavy chain mutants in the nematode Caenorhabditis elegans.
- An internal deletion mutant of a myosin heavy chain in Caenorhabditis elegans.
- Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.
- Protein structural domains in the Caenorhabditis elegans unc-54 myosin heavy chain gene are not separated by introns.
- Structures of spontaneous deletions in Caenorhabditis elegans.