The H+-ATPase in the Plasma Membrane of Saccharomyces cerevisiae Is Activated during Growth Latency in Octanoic Acid-Supplemented Medium Accompanying the Decrease in Intracellular pH and Cell Viability

AUTOR(ES)

Viegas, Cristina A.

FONTE

American Society for Microbiology

RESUMO

Saccharomyces cerevisiae plasma membrane H+-ATPase activity was stimulated during octanoic acid-induced latency, reaching maximal values at the early stages of exponential growth. The time-dependent pattern of ATPase activation correlated with the decrease of cytosolic pH (pHi). The cell population used as inoculum exhibited a significant heterogeneity of pHi, and the fall of pHi correlated with the loss of cell viability as determined by plate counts. When exponential growth started, only a fraction of the initial population was still viable, consistent with the role of the physiology and number of viable cells in the inoculum in the duration of latency under acid stress.

Documentos Relacionados

• ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.
• Role of the Plasma Membrane H+-ATPase in K+ Transport.
• Dominant lethal mutations in the plasma membrane H(+)-ATPase gene of Saccharomyces cerevisiae.
• Seasonal Changes of Plasma Membrane H+-ATPase and Endogenous Ion Current during Cambial Growth in Poplar Plants1
• H+-ATPase Activity from Storage Tissue of Beta vulgaris1: IV. N,N′-Dicyclohexylcarbodiimide Binding and Inhibition of the Plasma Membrane H+-ATPase