The H+/Cl- Symporter in Root-Hair Cells of Sinapis alba (An Electrophysiological Study Using Ion-Selective Microelectrodes).
AUTOR(ES)
Felle, H. H.
RESUMO
In root-hair cells of Sinapis alba, cytosolic pH, cytosolic [Cl-], membrane potential, and membrane resistance have been measured to investigate proton-driven Cl- transport across the plasma membrane. Rapid lowering of the external pH transiently increased cytosolic [Cl-] and acidified the cytoplasm. To an abrupt increase in external [Cl-] the cells reacted with a rapid initial depolarization and a subsequent slower hyperpolarization, which was accompanied by an increase in cytosolic [Cl-] and [H+]. These results are indicative of an nH+/Cl- symport with n > 1. Simultaneous recording of the membrane potential, the proton motive force, cytosolic pH, and cytosolic [Cl-] reveals that kinetically this Cl- transport depends on the pH gradient across the plasma membrane rather than on the membrane potential.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=159640Documentos Relacionados
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