The hemimethylated replication origin of Escherichia coli can be initiated in vitro.

AUTOR(ES)

Boye, E

RESUMO

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

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