The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region
AUTOR(ES)
Villa, Lina Marcela, Guhl, Felipe, Zabala, Daniel, Ramírez, Juan David, Urrea, Daniel Alfonso, Hernández, Diana Carolina, Cucunubá, Zulma, Montilla, Marleny, Carranza, Julio César, Rueda, Karina, Trujillo, Jorge Eduardo, Vallejo, Gustavo Adolfo
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2013-11
RESUMO
A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruziI to distinguish TcIDOMfrom sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.
Documentos Relacionados
- RNA-protein complexes mediate in vitro capping of the spliced-leader primary transcript and U-RNAs in Trypanosoma cruzi.
- Trypanosoma brucei spliced-leader RNA methylations are required for trans splicing in vivo.
- Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
- Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction.
- Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome†