The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants
AUTOR(ES)
Isaac, Peter G.
RESUMO
The single copy of the gene for cytochrome c oxidase subunit I (COX I) present in the mitochondrial genome of fertile maize (Zea mays L.) is encoded by a continuous open reading frame of 1584 nucleotides. The predicted polypeptide encoded by the gene has a mol. wt. of 58 219 daltons and shows >60% amino acid sequence homology with the corresponding fungal and animal polypeptides. Two major transcripts of 2400 and 2300 nucleotides can be detected and the 5' end of the larger transcript maps to a sequence from −161 to −153 (relative to the initiator codon) which shows high homology to the yeast mitochondrial promoter. In mitochondrial DNA from the S male-sterile cytoplasm of maize, which also characteristically contain two low mol. wt. linear DNAs (S1 and S2), rearrangements just 5' (at −175) to the COX I gene, generate additional DNA restriction fragments containing entire copies of the gene. These rearrangements involve a sequence identical to the terminal 186 bp of the 208-bp inverted repeat sequence found at either end of the S1 and S2 DNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554395Documentos Relacionados
- Molecular characterization of the Escherichia coli htrD gene: cloning, sequence, regulation, and involvement with cytochrome d oxidase.
- The sequence of the gene for cytochrome c oxidase subunit I, a frameshift containing gene for cytochrome c oxidase subunit II and seven unassigned reading frames in Trypanosoma brucei mitochrondrial maxi-circle DNA.
- Nucleotide sequence of the cytochrome oxidase subunit I gene from rice mitochondria.
- Cloning, DNA Sequence, and Expression of the Rhodobacter sphaeroides Cytochrome c2 Gene
- Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides cytochrome c2 gene.