The mechanism of action of tRNA methylases studied with immobilized tRNAs.

AUTOR(ES)

Gambaryan, A S

RESUMO

Each of the individual tRNAs immobilized on aminohydroxybutyl-cellulose (ABC) through their oxidized 3'-terminal binds affinitively all methylases present in the enzyme extract irrespective of whether this tRNA will be involved in the following step of methylation or not. These data allow to suggest that (a) the formation of a methylase-tRNA complex and the catalytic act of methylation are indeed autonomous processes and (b) the first step of interaction between tRNAs and tRNA methylases is rather unspecific and consists in the recognition of the whole class of tRNA molecules.

Documentos Relacionados

• Sequence of Trypanosoma brucei tRNA genes encoding cytosolic tRNAs.
• Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs.
• Evolution of tRNAs and tRNA genes in Acholeplasma laidlawii.
• Wobble modification differences and subcellular localization of tRNAs in Leishmania tarentolae: implication for tRNA sorting mechanism
• A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs.