The mechanism of production of multiple mRNAs for human glycophorin A.
AUTOR(ES)
Hamid, J
RESUMO
The major sialoglycoprotein in the human red cell surface membrane, glycophorin A is encoded by a single gene. However, this gene gives rise to three species of glycophorin A mRNA of sizes about 1.0, 1.7 and 2.8 kilobases in reticulocytes, foetal liver cells and erythroleukaemic K562 cells. In an investigation of how the three mRNAs originated, we showed by primer extension analysis that all three mRNAs in K562 cells had identical 5' termini and, by nucleotide sequencing of correlated cDNAs, that they had identical coding regions, except for the well-known glycophorin AM-AN polymorphism. However, we found also by sequencing the cDNAs that the mRNAs apparently differed from each other in the lengths of their 3' untranslated regions. This was confirmed by Northern blot analysis which also provided evidence that the three mRNAs originated by use of different polyadenylation signals of which seven were found in the longest cDNA we analyzed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332322Documentos Relacionados
- Human serum amyloid A. Three hepatic mRNAs and the corresponding proteins in one person.
- Multiple mRNAs for human alcohol dehydrogenase (ADH): developmental and tissue specific differences.
- Three rat preprotachykinin mRNAs encode the neuropeptides substance P and neurokinin A.
- Characterisation of mRNAs encoding the precursor for human apolipoprotein CI.
- Analysis of chimeric mRNAs derived from the STE3 mRNA identifies multiple regions within yeast mRNAs that modulate mRNA decay.