The methylation state of 2 muscle-specific genes: restriction enzyme analysis did not detect a correlation with expression.

AUTOR(ES)

Shani, M

RESUMO

To examine the possible role of DNA methylation in the modulation of expression of genes involved in the differentiation of muscle cells, we compared the methylation state of a number of CpG sites in the rat skeletal muscle actin and myosin light chain 2 genes, in muscle and nonmuscle cells, and in proliferating myoblasts and differentiated myotubes of the myogenic cell line L8. No correlation was detected between the state of methylation of these sites and the expression of the two genes. Essentially the same pattern of DNA methylation was observed, in the sites examined, in DNA from muscle, kidney and stomach. In DNA extracted from cultures of proliferating mononucleated myoblasts, as well as from differentiated multinucleated fibers of the myogenic cell line L8, the two genes were more methylated than in other tissues.

Documentos Relacionados

• Murine muscle-specific enolase: cDNA cloning, sequence, and developmental expression.
• The MRF4 activation domain is required to induce muscle-specific gene expression.
• Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression.
• Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression.
• Muscle-specific expression of SRF-related genes in the early embryo of Xenopus laevis.