The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid: A Double Labeling Determination 1
AUTOR(ES)
Walker, Graham C.
RESUMO
Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N6-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with 3H and in the 8-position of the purine ring with 14C. The 3H/14C ratio of N6-benzyladenosine recovered from labeled tobacco callus transfer RNA preparations was compared with the corresponding ratio for N6-benzyladenine supplied in the medium. By this means, with suitable controls, the incorporation of the synthetic cytokinin, N6-benzyladenine, into the transfer RNA of tobacco callus tissue was shown to involve the intact moiety. The observed level of incorporation was up to one N6-benzyladenosine molecule per 104 transfer RNA molecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=366592Documentos Relacionados
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