The nucleotide sequence of Bacillus subtilis tRNA genes.
AUTOR(ES)
Yamada, Y
RESUMO
Clones carring Bacillus subtilis tRNA genes were isolated from a lambda 816 library. A recombinant phage lambda 816-BS83 which was hybridized effectively with unfractionated tRNA probes contained a 3-kb fragment. By a Southern's blot analysis, it was found that tRNA genes were located in Eco RI-Hinc II region of this fragment. Sequence determination revealed the presence of a cluster of four tRNA genes in this region. The gene organization was as follows: tDNALys-9bp-tDNAGlu-81bp-tDNAAsp-30bp-tDNAPhe. The RNA sequences expected from tDNALys and tDNAPhe were identical with the reported RNA sequences. Two tRNA genes, tDNALys and tDNAAsp encoded the CCA sequence of 3'-terminal region, but the other two, tDNAGlu and tDNAPhe did not. A promoter-like sequence which corresponds to the sigma 55-recognition site was found in a region about 100bp upstream from tDNALys.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=325946Documentos Relacionados
- The nucleotide sequence of phenylalanine tRNA from Bacillus subtilis
- Nucleotide sequence of tryptophan tRNA from Bacillus subtilis.
- Nucleotide sequence of threonine tRNA from Bacillus subtilis.
- Nucleotide sequence of a lysine tRNA from Bacillus subtilis.
- Nucleotide sequence of non-initiator methionine tRNA from Bacillus subtilis.
